{"id":"a1022b84-dc35-44e4-bd8d-a1d0000b889e","title":"青藏高原土壤细菌多样性调查（V1.0）（2015）","title_en":"Bacteria distribution in Tibetan soils (version 1.0) (2015)","serial":"地球大数据科学工程专项时空三极环境项目","user_limit":"为尊重知识产权、保障数据作者的权益、扩展数据中心的服务、评估数据的应用潜力，请数据使用者在使用数据所产生的研究成果中（包括公开发表的论文、论著、数据产品和未公开发表的研究报告、数据产品等成果），明确注明数据来源和数据作者。对于转载（二次或多次发布）的数据，作者还须注明原始数据来源。","instructions":"","description":"青藏高原土壤细菌多样性数据集提供了青藏高原土壤表层（0-2厘米）微生物分布特征。样品采集时间为2015年7月1日至7月15日，包含草甸，草原，荒漠3种生态系统。土壤样品用冰袋保存，运回北京青藏高原研究所生态实验室。土壤DNA通过MO BIO PowerSoil DNA试剂盒提取。土壤表层样品采集后用液氮保存，运回悉尼实验室，通过FastPrep DNA试剂盒提取。提取后的DNA样品使用515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 909r (5'-GGACTACHVGGGTWTCTAAT-3')扩增16S rRNA基因片段。扩增后的片段通过Illumina Miseq PE250方式测序，原始数据通过Mothur软件分析。首先去除测序质量不佳序列，之后进行排序并去除嵌合体序列。之后计算序列之间相似度，相似度在97%以上的序列聚类为一个OTU，并定义OTU代表序列。OTU代表序列通过与Silva数据库进行比对，在可靠性大于>80%的情况下鉴定到属一级水平。本数据系统的比较了青藏高原微生物的多样性，对研究微生物在青藏高原的分布具有重大意义。","doi":"10.11888/Ecol.tpe.00000076.file","projection":"","language":"zh","share_type":"online","east":107.0,"west":67.0,"south":25.0,"north":45.0,"scale":null,"crs":"","filesize":3.0,"share_policy":"a","ts_created":"2018-02-16T22:05:43+08:00","doi_type":"","ts_updated":"2021-04-19T07:35:59.290814+08:00","time_description":"","datas_group":3,"time_description_en":"","data_format":"txt","project":[10,11],"specials":["1832ecb3-70d0-4b5e-bdb5-2e9ff4ad1efe","f2a78cde-1c98-4241-94dc-7532eba32b80"],"cite":"王磊、计慕侃. 青藏高原2015年7月土壤细菌多样性调查（V1.0）. 时空三极环境大数据平台. 2018","review_status":5,"review_invite":[],"review_editor":{"id":21057,"last_login":"2022-04-02T11:43:37.753860+08:00","username":"gecm","email":"gechm@lzb.ac.cn","is_active":true,"is_staff":true,"user_name":"盖春梅"},"data_sources":"","temporal_resolution":"","spatial_resolution":"","license":"Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","fund":[5],"subtopics":{"metadata":"a1022b84-dc35-44e4-bd8d-a1d0000b889e","three_pole":"XDA19070304","third_pole":null},"username":"计慕侃","review_time":null,"protected_duration":6,"protected_describe":null,"is_important":false,"hit_count":2886}