{"id":"ba7cef8e-2888-4e4e-8c59-b89a9e2bb66a","title":"青藏高原冰川微生物菌种资源（V1.0）（2010-2018）","title_en":"Bacteria strain resource database of the Tibetan Plateau (version 1.0) (2010-2018)","serial":"地球大数据科学工程专项时空三极环境项目","user_limit":"为尊重知识产权、保障数据作者的权益、扩展数据中心的服务、评估数据的应用潜力，请数据使用者在使用数据所产生的研究成果中（包括公开发表的论文、论著、数据产品和未公开发表的研究报告、数据产品等成果），明确注明数据来源和数据作者。对于转载（二次或多次发布）的数据，作者还须注明原始数据来源。","instructions":"","description":"青藏高原冰川细菌资源库数据集提供了刘勇勤实验组在2010-2018年间分离的青藏高原7条冰川（珠峰东绒布冰川，天山一号冰川，古里雅冰川，老虎沟冰川，木孜塔格冰川，七一冰川和玉珠峰冰川），向述荣分离的马兰冰川和张新芳分离的普若岗日冰川的细菌16S核糖体RNA基因序列。冰川样品采集后带回北京青藏高原院研究所生态实验室和兰州冰冻圈国家实验室，涂布平板后于不同温度下（4-25摄氏度）培养20天-90天并挑取单菌落纯化。分离的细菌提取DNA后以27F/1492R引物扩增16S核糖体RNA基因片段，并使用Sanger法测序。16S核糖体RNA基因序列通过“Classifier”软件与RDP数据库进行比对，在可靠性大于>80%的情况下鉴定到属一级水平。\n本数据包含每条序列的16S核糖体RNA基因片段序列及冰川来源。与以高通量测序为基础的序列相比，本数据的序列长度更长，分类更准确，更好的服务于冰川微生物研究。","doi":"10.11888/Ecolo.tpdc.270074","projection":"","language":"zh","share_type":"online","east":97.75,"west":75.05,"south":27.98333333,"north":43.11666667,"scale":null,"crs":"","filesize":1.0,"share_policy":"a","ts_created":"2018-02-19T23:26:05+08:00","doi_type":"","ts_updated":"2021-04-19T22:50:38.305870+08:00","time_description":"","datas_group":3,"time_description_en":"","data_format":"txt","project":[10,11],"specials":["1832ecb3-70d0-4b5e-bdb5-2e9ff4ad1efe","f2a78cde-1c98-4241-94dc-7532eba32b80"],"cite":"王磊，计慕侃.青藏高原2010-2018年冰川微生物菌种资源（V1.0）.时空三极环境大数据平台.2018","review_status":5,"review_invite":[],"review_editor":{"id":21057,"last_login":"2022-04-02T11:43:37.753860+08:00","username":"gecm","email":"gechm@lzb.ac.cn","is_active":true,"is_staff":true,"user_name":"盖春梅"},"data_sources":"","temporal_resolution":"","spatial_resolution":"m","license":"Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","fund":[5],"subtopics":{"metadata":"ba7cef8e-2888-4e4e-8c59-b89a9e2bb66a","three_pole":"XDA19070304","third_pole":null},"username":"计慕侃","review_time":null,"protected_duration":6,"protected_describe":null,"is_important":false,"hit_count":2849}