{"id":"e1d98f93-5b6d-40c8-bd53-dbdd57af20fe","title":"南北极细菌分布特征（V1.0）(2005-2006)","title_en":"Bacteria distribution in the Arctic and Antarctic (version 1.0) (2005-2006)","serial":"地球大数据科学工程专项时空三极环境项目","user_limit":"为尊重知识产权、保障数据作者的权益、扩展数据中心的服务、评估数据的应用潜力，请数据使用者在使用数据所产生的研究成果中（包括公开发表的论文、论著、数据产品和未公开发表的研究报告、数据产品等成果），明确注明数据来源和数据作者。对于转载（二次或多次发布）的数据，作者还须注明原始数据来源。","instructions":"","description":"南北极细菌分布数据集提供了南北极细菌分布特征。样品采集时间为13/12/2005至8/12/2006，包含北极3个地区52个样品（Spitsbergen Slijeringa，Spitsbergen Vestpynten，及Alexandra Fjord_Highlands），南极5个地区171个样品(Mitchell Peninsula,Casey station main power house,Robinsons Ridge,Herring Island,Browning Peninsula)。土壤表层样品采集后用液氮保存，运回悉尼实验室，通过FastPrep DNA试剂盒提取。提取后的DNA样品使用27F (5'-GAGTTTGATCNTGGCTCA-3' and 519R (5'-GTNTTACNGCGGCKGCTG-3')扩增16S rRNA基因片段。扩增后的片段通过454方式测序，原始数据通过Mothur软件分析。首先去除测序质量不佳序列，之后进行排序并去除嵌合体序列。之后计算序列之间相似度，相似度在97%以上的序列聚类为一个OTU，并定义OTU代表序列。OTU代表序列通过与Silva数据库进行比对，在可靠性大于>80%的情况下鉴定到属一级水平。本数据系统的比较了南极东部以及北极微生物的多样性，对研究微生物在南北极的分布具有重大意义。","doi":"10.11888/GlaciolGeocryol.tpe.0000008.file","projection":"","language":"zh","share_type":"online","east":162.0,"west":69.16666667,"south":77.61666667,"north":28.21666667,"scale":null,"crs":"","filesize":1.0,"share_policy":"a","ts_created":"2018-02-13T22:12:03+08:00","doi_type":"","ts_updated":"2021-04-19T10:00:21.158801+08:00","time_description":"","datas_group":3,"time_description_en":"","data_format":"txt","project":[10,11],"specials":["b4b306da-eab1-47ef-ae5d-be2556298efd","5f218875-5eed-4939-8208-7bf54c573e42"],"cite":"王磊,计慕侃.南北极细菌分布特征（V1.0）.时空三极环境大数据平台.2018","review_status":5,"review_invite":[],"review_editor":{"id":21057,"last_login":"2022-04-02T11:43:37.753860+08:00","username":"gecm","email":"gechm@lzb.ac.cn","is_active":true,"is_staff":true,"user_name":"盖春梅"},"data_sources":"","temporal_resolution":"","spatial_resolution":"","license":"Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","fund":[5],"subtopics":{"metadata":"e1d98f93-5b6d-40c8-bd53-dbdd57af20fe","three_pole":"XDA19070304","third_pole":null},"username":"计慕侃","review_time":null,"protected_duration":6,"protected_describe":null,"is_important":false,"hit_count":2008}